厦 门 大 学
教 学 进 度 表
(2022-2023学年第1学期)
课程名称遗传与分子生物学实验 学分3 上课系、专业、年级 生科院2020级
教师姓名 章军 等 职称 副教授 邮箱jzhang@xmu.edu.cn 电话13950189238
周数 (按校历) | 各章节教学内容纲要 | 课时安排(学时) | 主讲人 | 备注 | ||||
授课 | 讨论 | 实验/上机 | 实践 | 其他 | ||||
1 | 第一章绪论,课程介绍,实验室规则、实验安全教育、课程考试评分细节等 | 6 |
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| 章军 |
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2 | 实验一果蝇的饲养和观察 | 1 |
| 4 |
| 1 | 章军、顾颖 | 作业1 |
3 | 创新大实验开题 果蝇诱变 |
| 8 |
|
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| 章军、顾颖 | 作业1 |
4 | 实验二细菌转导 | 1 |
| 4 |
| 1 | 章军、顾颖 | 作业1 |
5 | 实验三转座子引起的插入突变 | 1 |
| 4 |
| 1 | 章军、顾颖 | 作业1 |
6 | 实验四质粒DNA的制备 | 1 |
| 4 |
| 1 | 杨玉荣、王亚梅 | 作业1 |
7 | 实验五质粒DNA的酶切 | 1 |
| 4 |
| 1 | 杨玉荣、王亚梅 | 作业1 |
8 | 实验六DNA片段从凝胶中分离和连接 | 1 |
| 4 |
| 1 | 杨玉荣、王亚梅 | 作业1 |
9 | 实验七 质粒DNA转化 | 1 |
| 4 |
| 1 | 杨玉荣、王亚梅 | 作业1 |
10 | 实验八外源基因在大肠杆菌中高效表达 | 1 |
| 4 |
| 1 | 杨玉荣、王亚梅 | 作业1 |
11 | 实验九真核细胞基因组提取 | 1 |
| 4 |
| 1 | 黄秋英、程通 | 作业1 |
12 | 实验十DNA纯化及鉴定 | 1 |
| 4 |
| 1 | 黄秋英、程通 | 作业1 |
13 | 实验十一 PCR技术 | 1 |
| 4 |
| 1 | 黄秋英、程通 | 作业1 |
14 | 实验十二 细胞转染 | 1 |
| 4 |
| 1 | 黄秋英、程通 | 作业1 |
15 | 创新大实验总结 果蝇诱变 |
| 8 |
|
|
| 章军、顾颖 | 作业1 |
17 | 复习、期末考试 | 2 |
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| 章军 | 期末考 |
合计 | 96 |
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制定者: 审定者: 制定时间:2022 年9月1日
Teaching Schedule
(Academic year 2022-2023,Semester1)
Course name Genetic&Mol. Biology Credit 3 Dept., Class School of Life Sciences, 2020
Instructor name Zhang Jun etc. Title Associate Prof. Email jzhang@xmu.edu.cn Tel:13950189238
Week (University Calendar) | Content | Learning Hours | Note | |||||
Lecture | Tutorial | Experiment | Practical | Others | ||||
1 | Introduction of course | 6 |
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| Zhang |
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2 | Breeding and hybridization experiment of Drosophila | 1 |
| 4 |
| 1 | Zhang&Gu | 1 assignment |
3 | Mutation of Drosophila |
| 8 |
|
|
| Zhang&Gu | 1 assignment |
4 | Bacterial transduction | 1 |
| 4 |
| 1 | Zhang&Gu | 1 assignment |
5 | Transposon insertion mutations experiment | 1 |
| 4 |
| 1 | Zhang&Gu | 1 assignment |
6 | Plasmid DNA preparation | 1 |
| 4 |
| 1 | Yang&Wang | 1 assignment |
7 | Plasmid DNA restriction endonuclease cleave | 1 |
| 4 |
| 1 | Yang&Wang | 1 assignment |
8 | DNA fragments isolated from the gel and DNA ligation experiment | 1 |
| 4 |
| 1 | Yang&Wang | 1 assignment |
9 | Plasmid DNA transformation | 1 |
| 4 |
| 1 | Yang&Wang | 1 assignment |
10 | Expression of foreign genes in Escherichia coli | 1 |
| 4 |
| 1 | Yang&Wang | 1 assignment |
11 | Extraction of eukaryotic genome DNA | 1 |
| 4 |
| 1 | Huang&Chen | 1 assignment |
12 | DNA purification and identification | 1 |
| 4 |
| 1 | Huang&Chen | 1 assignment |
13 | Polymerase chain reaction amplify DNA experiment | 1 |
| 4 |
| 1 | Huang&Chen | 1 assignment |
14 | Gene transfection of eukaryotic cell | 1 |
| 4 |
| 1 | Huang&Chen | 1 assignment |
15 | Summary of Drosophila mutation |
| 8 |
|
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| Zhang&Gu | 1 assignment |
17 | review and final exam | 2 |
|
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| Zhang | Final exam |
Total | 96 |
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Filled out by Approved by: Date: 2022.9