Course name

Genetic and molecular biology experiment

Course group

Genetic & Development

Course code

130110010029

Category code

BIOL

Course can be   shared with

Biology department student

Course type

□Basic Common Courses 公共基本课程

□General Education Courses 通识教育课程

■Disciplinary General Courses 学科通修课程

□Specialized Courses 学科或专业方向性课程

□Other其它教学环节

□Disciplinary Courses专业课程

□Electives Courses任选课程

□Extra Courses 方案外课程

Course   focus

□Lecture

■Experiment

□Skill-training

□Practical

□Lecture +   Experiment

□Lecture +   Practical

Credit

Total learning hours

Lecture

Experiment

Practical

2

96

24

72

0

Prerequisites

Genetic，Molecular Biology

1.Course   description

This course is divided   into two parts: genetics experiments and molecular biology experiments.   Genetics experiment take advantage of model organism: Drosophila, nematodes,   and Escherichia coli for learning genetic manipulation, deepening   understanding of genetics model organism. Molecular Biology experiment   systematic include the extraction of DNA, enzyme cleave and ligation,   construction expression vector, transformation and expression foreign gene,   focus on learning the common molecular biology technologies.

2. Learning goals

Through the study and practice of genetics and molecular biology experiments, deepen   the understanding of genetics and molecular biology theories, learn   experimental methods and technologies, cultivate students' scientific spirit   and craftsman spirit, and strengthen the ability to solve biological   problems.

3.Moral education goals

Shape the   scientific spirit of rigorous thinking and seeking truth from facts.

Cultivate   patience + carefulness + curiosity and teamwork + innovation

4.Teaching approaches

Online and offline Hybrid   Teaching

5. Content outline of the course (including   moral education elements)

Chapter(Section or experimental item)

Content

Main point of moral education

Learning hours

Type of experimental item

Real experiment or virtual simulation 

experiment 

Requirement of experimental item

1.   Introduction

Course   introduction, laboratory rules, laboratory safety education, course exam   scoring details, etc.

Learn   pragmatic, scientific thinking and innovative ideas.

6

Demonstrative

Real experiment

Must do

2.Feeding and observation of fruit flies

Familiarize yourself with fruit flies and lay the foundation   for subsequent open experiments.

Learn about the history of the study of fruit flies, the   uncrowned king of the laboratory and the subject of six Nobel Prizes.

6

Verifiable

Real experiment

Must do

3.Fruit fly open experiment (opening topic)

Representatives of the group took the stage to introduce the   design of the Open Experiment

Innovation is at the heart of biological research, and   experience it firsthand.

6

Designed

Real experiment

Must do

4.Bacterial transduction

The transfer of foreign genes is mediated by phage infection   and the principles of experimental design are learned.

Knowing what is true and knowing why, scientific experimental   design requires rigorous logic.

6

Verifiable

Real experiment

Must do

5.Transposon-induced insertion mutations

Learn about the history of transposon discovery, understand   transposon concepts, and learn experimental verification design for bacterial   insertion mutations.

For the subversive discovery of transposons, the design of   experimental materials and controls is crucial, and the ingenuity of   experimental design is appreciated.

6

Verifiable

Real experiment

Must do

6.Preparation and digestion of plasmid DNA

Understand the characteristics of plasmid DNA and learn   several methods and principles of extracting plasmid DNA.

The microscopic world should use microscopic technical   methods, which are similar to macroscopic ones. Designing genetic engineering   experiments artificially reflects human ingenuity.

6

Research

Real experiment

Must do

7.DNA fragments are separated and ligated from the gel

Learn how to run DNA and gel recycle.

Microsurgery at the molecular level, gene editing techniques

6

Research

Real experiment

Must do

8.Plasmid DNA transformation

Master the principles and methods of various plasmids DNA   transformation.

Comparing multiple methods, technological progress must be   based on understanding principles. The prerequisite for improving efficiency   is a true understanding of the principle and essence.

6

Comprehensive

Real experiment

Must do

9.Foreign genes are efficiently expressed in E. coli

Learn the method of induced expression of foreign genes, and   master the technology of protein SDS-PAGE to verify the expression products.

Compare the perfect experimental experience, and the   realization of the final goal needs to be guided step by step.

6

Research

Real experiment

Must do

10.Eukaryotic cell genome extraction

Learn   the extraction principle and preparation method of genomic DNA from plant   leaves.

The   debate over whether genetic material is DNA or protein is a nuanced one, bold   assumptions, and rigorous logical arguments.

6

Research

Real experiment

Must do

11.DNA purification and identification

Understand the physical   and chemical properties of DNA, master several methods of DNA purification,   and learn the principles and methods of DNA purity identification.

In 1953, the analysis of   DNA structure opened the era of molecular biology research. Great discoveries   depend on interdisciplinary intersectionality and materialism.

6

Research

Real experiment

Must do

12.PCR technology

Understand the principle   of PCR technology, master the use of PCR instruments and technical   applications.

The biotechnology revolution   brought about by the invention of PCR technology. Technology is a weapon for   scientific research, and a deep understanding of principles can lead to   innovation.

6

Designed

Real experiment

Must do

13.Cell transfection

Understand the principles and methods of liposome transfection   of animal cells.

Introducing the contributions and applications of HeLa cells.   Cancer cells are also useful, and it is necessary to look at the problem   dialectically.

6

Verifiable

Real experiment

Must do

14. Open big experiment summary report

The representative of the group reported the summary of the open   experimental research since the semester, and the comments of students and   teachers.

The scientific spirit of curiosity and skepticism, innovation is   the essence of research. By allowing students to experience a real and   complete scientific research process, students are guided to love science.

6

Research

Real experiment

Must do

Total

96

6. Assessment methods   and requirements

Assessment   criteria: Normal experiment 30%+ open design experiment 20%+ SPOC 30%+ Final   exam 30%.

7.Textbooks/   References

Genetic and   molecular biology experiment notes (self-edited)

8.Major   literature or related databases

Genetic and molecular biology experiment   digital course, Zhang J editor, Higher education publishing house, 2019.10,   ISBN：978-7-89510-547-8

9.Website

http://www.icourse163.org/spoc/learn/XMU-1002235002?tid=1002350003

10.Others

e.g. classroom   rules of conduct

Filled out by:        Approved by:     Date:  2022.9