周数

(按校历)

各章节教学内容纲要

课时安排（学时）

主讲人

备注

授课

讨论

实验/上机

实践

其他

第4周周一上午

秀丽线虫作为模式生物的历史回顾及优势

2

俞勇

作业1

第4周周一下午

秀丽线虫培养及基本操作

3

俞勇

实验考核1

实验报告1

第4周周二

秀丽线虫突变体辨识、同步化及遗传杂交(1)

5

俞勇

实验考核2

实验报告2

第4周周三上午

秀丽线虫的形态与解剖结构

2

俞勇

作业2

第4周周三下午

秀丽线虫发育过程及形态、解剖结构观察

3

俞勇

实验考核3

实验报告3

第4周周四上午

秀丽线虫的基因与发育等内容

2

罗时成

作业3

第4周周四下午

秀丽线虫的RNAi及遗传杂交(2)

3

俞勇

实验报告4

第4周周五

冻存，Single worm PCR and Genotyping

5

俞勇

实验报告5

第5周周一

RNAi结果分析和脂肪染色，遗传杂交(3)

5

俞勇

实验报告6

第5周周二上午

亚洲秀丽线虫大会线上报告

2

俞勇

第5周周二下午

荧光蛋白在生物学中的应用(基因表达调控及蛋白质在细胞中定位研究)

3

俞勇

实验报告7

第5周周三上午

秀丽线虫研究中常用的实验技术及方法

2

作业4

第5周周四-五上午

文献报告与讨论

2

2

俞勇

第5周周三-五下午

线虫实验室参观和显微注射

9

俞勇

实验报告8

合计

10

4

36

Week

(University   Calendar)

Content

Learning   Hours

Instructor

Note

Lecture

Tutorial

Experiment

Practical

Others

4^th   Week, Mon. AM.

Historical review and advantages of C.   elegans as a model organism

2

Yong Yu

Assignment   1

4^th   Week, Mon. PM.

Culture and basic operation of C.   elegans

3

Yong Yu

Experimental examination 1; Experimental   report 1

4^th   Week, Tue.

Mutant identification, synchronization and genetic cross of C. elegans (1)

5

Yong Yu

Experimental examination 2; Experimental   report 2

4^th   Week, Wed. AM.

Morphology and anatomical structure of C. elegans

2

Yong Yu

Assignment 2

4^th   Week, Wed. PM.

Observation on developmental process, morphology and anatomical structure   of C. elegans

3

Yong Yu

Experimental examination 3; Experimental   report 3

4^th   Week, Thu. AM

Genes and development of C.   elegans

2

SJ Lo

Assignment 3

4^th   Week, Thu. PM

C. elegans RNAi and   genetic cross (2)

3

Yong Yu

Experimental report 4

4^th   Week, Fri.

Freezing, single worm PCR and Genotyping

5

Yong Yu

Experimental report 5

5^th   Week,

Mon.

RNAi result analysis, fat staining and genetic cross (3)

5

Yong Yu

Experimental report 6

5^th   Week,

Tue.   AM

Asia Pacific Worm Meeting, online seminar

2

Yong Yu

5^th   Week,

Tue.   PM

Application of fluorescent protein in Biology (gene expression   regulation and protein localization in cells)

3

Yong Yu

Experimental report 7

5^th   Week,

Wed.   AM

Experimental techniques and methods commonly used in the study of C. elegans

2

Yong Yu

Assignment 4

5^th   Week,

Thu   to Fri. AM

Journal presentation and discussion

2

2

Yong Yu

5^th   Week,

Wed.   to Fri. PM

C. elegans lab visit and   microinjection

9

Yong Yu

Experimental report 8

Total

10

4

36