厦 门 大 学
教 学 进 度 表
(2021-2022学年第3学期)
课程名称 生物技术实验 学分 2 上课系、专业、年级 生科院2019级
教师姓名 李勤喜,林舒勇 职称 教授/教授 邮箱liqinxi@xmu.edu.cn, linsy@xmu.edu.cn
电话13850006392,15985860458
周数 | 各章节教学内容纲要 | 课时安排(学时) | 主讲人 | 备注 | ||||
授课 | 讨论 | 实验/上机 | 实践 | 其他 | ||||
4 | 哺乳动物细胞培养 | 1 | 1 | 4 |
|
| 李勤喜 |
|
4 | 细胞总RNA 的制备 | 1 | 1 | 4 |
|
| 李勤喜 |
|
4 | mRNA反转录成cDNA | 1 | 1 | 4 |
|
| 林舒勇 |
|
4 | PCR扩增目的基因 | 1 | 1 | 4 |
|
| 林舒勇 |
|
4 | 哺乳动物细胞基因转染 | 1 | 1 | 4 |
|
| 林舒勇 |
|
4 | 1 | 2 | 9 |
|
| 林舒勇 |
| |
5 | Western 印迹法检测目的基因的表达 | 2 | 2 | 10 |
|
| 李勤喜 |
|
5 | 荧光蛋白作为报告基因研究蛋白的表达和定位 | 2 | 2 | 10 |
|
| 李勤喜 |
|
5 | 答疑 |
|
|
|
|
| 李勤喜 林舒勇 |
|
合计 | 70 |
|
|
|
|
|
|
制定者:李勤喜 审定者:王勤 制定时间:2022年5月28 日
Xiamen University
Teaching Schedule
(Academic year 2021-2022,Semester 3)
Course name Biotechnique Experiments Credit 2 Dept., Class school of life sciences, 2019
Instructor name Qinxi Li, Shuyong Lin Title Professor/ Professor
Email liqinxi@xmu.edu.cn, linsy@xmu.edu.cn Tel 13850006392, 15985860458
Week (University Calendar) | Content | Learning Ho rs | Instructor | Note | ||||
Lecture | Tutorial | Experiment | Practical | Others | ||||
1 | Mammalian cell culture | 1 | 1 | 4 |
|
| Qinxi Li |
|
1 | RNA extraction | 1 | 1 | 4 |
|
| Qinxi Li |
|
1 | Reverse transcription | 1 | 1 | 4 |
|
| Shuyong Lin |
|
1 | PCR technology | 1 | 1 | 4 |
|
| Shuyong Lin |
|
1 | Gene transfection in mammalian cell | 1 | 1 | 4 |
|
| Shuyong Lin |
|
1 | Study of proteins interactions using co-immunoprecipitation | 1 | 2 | 9 |
|
| Shuyong Lin |
|
2 | Analysis of gene expression by Western blot | 2 | 2 | 10 |
|
| Qinxi Li |
|
2 | Study of proteins expression and co-localization by using fused fluorescent proteins proteins
| 2 | 2 | 10 |
|
| Qinxi Li |
|
2 | Review |
|
|
|
|
| Qinxi Li Shuyong Lin |
|
Total | 70 |
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
Filled out by: Qinxi Li Approved by: Qin Wang Date: 2022.5.28