厦 门 大 学
教 学 进 度 表
(2022－2023学年第2学期)
课程名称 细胞与显微技术实验 学分 3 上课系、专业、年级 生科院2022级
教师姓名 江良荣 职称 邮箱lrjiang108@xmu.edu.cn 电话 13599509420
周数 (按校历) | 各章节教学内容纲要 | 课时安排（学时） | 主讲人 | 备注 | ||||
授课 | 讨论 | 实验/上机 | 实践 | 其他 | ||||
3 | 相差显微镜与暗视野显微镜的使用 | 1 |
| 5 |
|
| 余娴文 靳全文 | 小测1次、作业1次 |
4 | 细胞荧光染色观察与荧光显微镜的使用；动物细胞线粒体的超活染色与油镜观察 | 1 |
| 5 |
|
| 余娴文 靳全文 | 小测1次、作业1次 |
5 | 细胞核与线粒体的分级分离（差速离心） | 1 |
| 5 |
|
| 靳全文 江良荣 | 小测1次、作业1次 |
6 | 兔血细胞计数及细胞大小的测量 | 1 |
| 5 |
|
| 靳全文 江良荣 | 小测1次、作业1次 |
7 | 叶绿体的分离与观察（密度梯度离心）（直接荧光与间接荧光的比较） | 1 |
| 5 |
|
| 靳全文 刘 敏 | 小测1次、作业1次 |
8 | 植物细胞骨架的观察 | 1 |
| 5 |
|
| 靳全文 刘 敏 | 小测1次、作业1次 |
9 | 细胞Barr body 的观察 | 1 |
| 5 |
|
| 刘 敏黄秋英 | 小测1次、作业1次 |
10 | 免疫荧光染色观察肿瘤细胞骨架 | 1 |
| 5 |
|
| 刘 敏黄秋英 | 小测1次、作业1次 |
11 | 细胞染色体的制备与染色 | 1 |
| 5 |
|
| 黄秋英 刘 敏 | 小测1次、作业1次 |
12 | 血红细胞通透性与细胞融合的观察 | 1 |
| 5 |
|
| 黄秋英 刘 敏 | 小测1次、作业1次 |
13 | 细胞凝集反应 | 1 |
| 5 |
|
| 黄秋英 江良荣 | 小测1次、作业1次 |
14 | 石蜡切片技术创新性实验 （一）取材（阿兹夫定喂养小鼠）、固定、洗涤、脱水 （二）透明、透蜡、包埋 | 1 |
| 5 |
|
| 江良荣叶 军 | 小测1次、作业1次 |
15 | （三）切片、贴片；实验十四、冰冻切片技术 | 1 |
| 5 |
|
| 江良荣叶 军 | 小测1次、作业1次 |
16 | （四）染色（分化与蓝化的组合分析）、封藏 | 1 |
| 5 |
|
| 江良荣叶 军 | 小测1次、作业1次 |
17 | 组织化学创新性实验——小鼠肝和胸腺糖元染色；Feulgen 反应 | 1 |
| 5 |
|
| 叶 军 余娴文 | 小测1次、作业1次 |
18 | 免疫组织化学创新性实验——小鼠脑和胸腺血管的DAB显色 | 1 |
| 5 |
|
| 叶 军 余娴文 | 小测1次、作业1次 |
19 | 复习、期末考试 |
|
|
|
|
|
| 期末考试 |
合计 | 16 |
| 80 |
|
|
|
| |
制定者： 审定者： 制定时间： 年 月 日
Xiamen University
Teaching Schedule
(Academic year 2022－2023，Semester 2)
Course name Cell and microscopic techniques Credit 3 Dept., Class School of Life Sciences, 2021
Instructor name Liangrong Jiang Title Email lrjiang108@xmu.edu.cn Tel 13599509420
Week (University Calendar) | Content | Learning Hours | Note | |||||
Lecture | Tutorial | Experiment | Practical | Others | ||||
3 | The usage of Phase contrast and dark field microscope | 1 |
| 5 |
|
| Xianwen Yu Quanwen Jin | 1 quiz and 1 assignment |
4 | Fluorescence staining and the usage of fluorescence microscopy; Animal cell mitochondria vital staining and oil microscopic observation |
| 5 |
|
| Xianwen Yu Quanwen Jin | 1 quiz and 1 assignment | |
5 | Fractionation of nuclei and mitochondria (differential centrifugation) | 1 |
| 5 |
|
| Quanwen Jin Liangrong Jiang | 1 quiz and 1 assignment |
6 | Cell count and size measurement of rabbit blood cells | 1 |
| 5 |
|
| Quanwen Jin Liangrong Jiang | 1 quiz and 1 assignment |
7 | Isolation and observation of chloroplasts (density gradient centrifugation) (Comparison of direct and indirect fluorescence) | 1 |
| 5 |
|
| Quanwen Jin Min Liu | 1 quiz and 1 assignment |
8 | Observation of cytoskeleton of plant cells | 1 |
| 5 |
|
| Quanwen Jin Min Liu | 1 quiz and 1 assignment |
9 | Observation of cell barr body | 1 |
| 5 |
|
| Min Liu Qiuying Huang | 1 quiz and 1 assignment |
10 | Observation of cytoskeleton by immunofluorescence | 1 |
| 5 |
|
| Min Liu Qiuying Huang | 1 quiz and 1 assignment |
11 | Preparation and staining of cell chromosome | 1 |
| 5 |
|
| Qiuying Huang Min Liu | 1 quiz and 1 assignment |
12 | Observation of the permeability and fusion of red blood cell | 1 |
| 5 |
|
| Qiuying Huang Min Liu | 1 quiz and 1 assignment |
13 | Cell agglutination | 1 |
| 5 |
|
| Qiuying Huang Liangrong Jiang | 1 quiz and 1 assignment |
14 | Paraffin sectioning (Innovation experiment) (1) specimen preparation (The mice were fed with the medicine, Azvudine), fixation , washing , dehydration (2) Clearing, infiltration and embedding | 1 |
| 5 |
|
| Liangrong Jiang Jun ye | 1 quiz and 1 assignment |
15 | (3) Sectioning; Frozen section technique (Innovation experiment) | 1 |
| 5 |
|
| Liangrong Jiang Jun ye | 1 quiz and 1 assignment |
16 | (4) Staining and storage (Innovation experiment) | 1 |
| 5 |
|
| Liangrong Jiang Jun ye | 1 quiz and 1 assignment |
17 | Histochemistry (Innovation experiment): Mouse liver and thymus glycogen staining (PAS reaction)；Mouse liver and thymus DNA staining (Feulgenreaction) | 1 |
| 5 |
|
| Jun ye Xianwen Yu | 1 quiz and 1 assignment |
18 | Immunohistochemical assay (Innovation experiment)：DAB staining of brain and thymus vessels in mice | 1 |
| 5 |
|
| Jun ye Xianwen Yu | 1 quiz and 1 assignment |
19 | review and final exam |
|
|
|
|
|
| Final exam |
Total | 16 |
| 80 |
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Filled out by Approved by: Date: