Course name

Cell and microscopic technique

Course group

Cell biology

Course code

130110010027

Category code

BIOL

Course can be   shared with

All major

Course type

□Basic Common   Courses

□General Education   Courses

RDisciplinary General   Courses

□Specialized Courses   

□Other

□Disciplinary   Courses

□Electives Courses

□Extra Courses

Course   focus

□Lecture

RExperiment

□Skill-training

□Practical

□Lecture +   Experiment

□Lecture +   Practical

Credit

Total learning hours

Lecture

Experiment

Practical

3

96

16

80

Prerequisites

None

1.Course   description

This course is   a major course of life science. The course is for sophomores or freshmen and   lasts for the second semester. The total credit is 3 and class hour is 96.   The main content of this course is to use the conventional research methods   of cell biology to carry out some primary validation or innovative   experiments. By learning this course, students will be able to master some   basic experimental skills, such as the techniques for microscopical slides,   various organelle separation methods, observation of cell and subcellular   structure, measurement of cell size, cell counting and immunohistochemical   experiments. Understand the application of various special microscopes in   cell biology research. Through the study of this course, students will   develop good experimental habits, cultivate standard experimental operation   and master common experimental techniques, which will lay a foundation for   future teaching and scientific research.

2. Learning   goals

Students are   required to master the basic knowledge and basic skills of cell and   microscopic technology, and cultivate good experimental habits. The students   should deeply understand the fundamentals of each experiment; master the   skills of using various related instruments and equipment, reagent   preparation, etc.; be proficient in experimental procedures; comprehensively   apply the knowledge learned to analyze and solve various problems in the   process of experiment. Students will also be appropriately involved in the   design of some innovative experiments to develop scientific research   thinking.

3.Moral education goals

Moral education goals: Inculcate the   concept of being kind to experimental animals, carry out humanistic care and   integrity education of experimental data. To appreciate and learn from the   scientific spirit and personality charm of great scientists who break through   conventions, have the courage to explore, and are rigorous and realistic.

4.Teaching   approaches

Mixed online and offline teaching model.   Teachers release preview tasks online in advance, and students use MOOC   resources for preview and quiz. On the basis of regular experiments, students   can make innovative designs for some experiments.

During the offline class, the teacher   tells the experiment fundamentals, experiment procedure and matters needing   attention, then the students do experiments and the teachers walk around and   instruct the students. After the class, the students write the experiment   report.

5. Content outline of the course   (including moral education elements)

Chapter(Section or experimental item)

Content

Main   point of moral education

Learning   hours

Type of   experimental item

Real experiment or virtual simulation

experiment   

Requirement   of experimental item

Exp. 1

The   usage of Phase contrast and dark field microscope

The   importance of good experimental habits to scientific research

6

Verification

Real experiment

Requirement

Exp. 2

Fluorescence   staining and the usage of fluorescence microscopy.

·           The development history of fluorescence   microscope which guides students to have a global view.

6

Verification

Real experiment

Requirement

Exp. 3

Animal   cell mitochondria vital staining and oil microscopic observation

Introduce   the concept of animal ethics and kindness to animals

Verification

Real experiment

Requirement

Exp. 4

Fractionation   of nuclei and mitochondria

 (differential centrifugation)

6

Comprehensiveness

Real experiment

Requirement

Exp. 5

Isolation   and observation of chloroplasts (density gradient centrifugation) (Comparison of direct and indirect fluorescence)

The   discovery process of chlorophyll fluorescence induction phenomenon, learning   the scientific spirit of rigorous exploration

6

Comprehensiveness

Real experiment

Requirement

Exp. 6

Observation   of plant cytoskeleton

6

Verification

Real experiment

Requirement

Exp. 7

Cell   count and size measurement of rabbit blood cells

6

Verification

Real experiment

Requirement

Exp. 8

Observation   of cell barr body

6

Verification

Real experiment

Requirement

Exp. 9

Fluorescence   microscopy of tumor cytoskeleton

The   idea of details determine the success or failure of the   experiment is integrated into the whole experimental teaching process

6

Verification

Real experiment

Requirement

Exp. 10

Preparation   and staining of cell chromosome

Introduce   the concept of animal ethics and kindness to animals

6

Verification

Real experiment

Requirement

Exp. 11

Observation   of the permeability and fusion of red blood cell

Emphasize   the importance of careful observation

6

Verification

Real experiment

Requirement

Exp. 12

cell   agglutination reaction

Introduce   the importance of patience in experiments

6

Verification

Real experiment

Requirement

Exp. 13

Paraffin   sectioning (Innovation experiment)

(1)    specimen   preparation (The mice were fed with the medicine, Azvudine), fixation , washing , dehydration

(2)    Clearing,   infiltration and embedding

6

Design

Innovation experiment

Requirement

Exp. 13

(3)    Sectioning   (Innovation experiment)

6

Verification

Innovation experiment

Requirement

Exp. 14

Frozen   section technique

Verification

Innovation experiment

Requirement

Exp. 13

(4)    Stainning   and storage (Innovation experiment)

The   discovery of Reich-Mssage staining method inspired scientific research to be   careful observation, good at discovery and brave in exploration

6

Comprehensiveness

Innovation experiment

Requirement

Exp. 15

Histochemistry   (Innovation experiment): Mouse liver   and thymus glycogen staining (PAS   reaction)

6

Verification

Innovation experiment

Requirement

Exp. 16

Histochemistry   (Innovation experiment): Mouse liver and   thymus DNA staining (Feulgenreaction)

Verification

Innovation experiment

Requirement

Exp. 17

Immunohistochemical   assay (Innovation experiment)：DAB   staining of brain  and thymus vessels in mice

6

Verification

Innovation experiment

Requirement

Total

96

6. Assessment methods and requirements

The score of experimental reports and   MOOC accounts for 50%,and operating test accounts for 50%

7.Textbooks/References

Teaching   materials of cell and microscopic techniques

8.Major   literature or related databases

9.Website

http://course.xmu.edu.cn/meol/jpk/course/layout/newpage/index.jsp?courseId=14718

http://www.icourse163.org/course/XMU-120746780

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