Course name

The model   organism Caenorhabditis elegans:   introduction and practice

Course group

Cell Biology

Course code

U10302300111

Category code

BIOL

Course can be   shared with

Bioscience; Biotechnology, Year 2 or 3

Course type

□Basic Common Courses 公共基本课程

□General Education Courses 通识教育课程

□Disciplinary General Courses 学科通修课程

√Specialized Courses 学科或专业方向性课程

□Other其它教学环节

□Disciplinary Courses专业课程

□Electives Courses任选课程

□Extra Courses 方案外课程

Course   focus

□Lecture

□Experiment

□Skill-training

□Practical

√Lecture + Experiment

□Lecture +   Practical

Credit

Total learning hours

Lecture

Experiment

Practical

2

50

14

36

0

Prerequisites

1.Course   description

This   course is a specialized elective course for undergraduates majoring in life   science. In the form of a small class, the course promotes the discussion and   interaction between teachers and students, and combines students' actual   operation. The main knowledge points of this course include: the biological   characteristics of C. elegans as a   model organism, the morphology and structure of C. elegans, the genes and development of C. elegans, and the experimental techniques and methods commonly   used in C. elegans research. The   basic contents of each part are as follows: 1. Biological characteristics of C. elegans as a model organism:   understand the common biological characteristics of model organisms, and   further understand the biological characteristics of C. elegans as a model organism through experiments; 2. Morphology   and structure of C. elegans:   understand how C. elegans adapt to   different living environments, and understand the morphology and anatomical   structure of different tissues and organs through experiments and   observation; 3. Genes and development of C.   elegans: understand that the individual development of C. elegans is regulated by genes, and   observe and understand the regulation mode of gene expression in the   experiment; 4. Experimental techniques and methods commonly used in the study   of C. elegans: understand the   experimental techniques and methods developed in the study of C. elegans, understand the scope and   limitations of the application of experimental techniques and methods, and   learn these useful techniques and methods in the experimental operation.

2. Learning goals

This course will   enable students to master the biological characteristics of Caenorhabditis elegans as a model   organism, be familiar with its morphology, structure, growth and development,   and understand the mechanism of gene expression regulation in the process of   growth and development, and comprehensively learn and understand the latest   research methods and experimental techniques of C. elegans. Through integrating theory with practice, this course   can cultivate students' scientific spirit and innovative consciousness. Students   can deeply understand the important role of model biology in life science research,   and provide scientific guidance for them to correctly select experimental   materials and technical methods in future research work.

3. Moral education goals

During the lecture courses, I will introduce some important   achievements and outstanding contributions made by Chinese scientists in the   field C. elegans in order to cultivate students' interest in science,   scientific spirit and national pride. In the experiment courses, students can   combine theoretical knowledge with practice through hands-on experiments, and   cultivate students' patience, carefulness, hands-on ability and interest in   scientific research.

4.Teaching approaches

This course adopts a variety of teaching   methods and forms, such as multimedia courseware explanation, classroom   discussion, experiments, students' flipping classroom journal presentation, etc., which is helpful for students   to master knowledge and methods and deepen their understanding in practice. Small   class facilitates the communication and interaction between teacher and   students.

5. Content outline of the course (including   moral education elements)

Chapter(Section or experimental item)

Content

Main point of moral education

Learning hours

Type of   experimental item

Real experimentor virtual simulation 

experiment 

Requirement of   experimental item

Lecture 1

Historical review and advantages of C.   elegans as a model organism

Introduce the important achievements and outstanding   contributions of Chinese scientists

2

Real experiment

Experiment 1

Culture and basic operation of C.   elegans

Cultivate students' care and patience

3

Validation

Real experiment

Mandatory

Experiment 2

Mutant identification, synchronization, and genetic cross of C. elegans (1)

Integrating theory   with practice

5

Comprehensiveness

Real experiment

Mandatory

Lecture 2

Morphology and anatomical structure of C. elegans

2

Experiment 3

Observation on developmental process, morphology and anatomical   structure of C. elegans

Integrating theory with practice

3

Comprehensiveness

Real experiment

Mandatory

Lecture 3

Genes and development of C.   elegans

2

Experiment 4

C. elegans RNAi and genetic cross (2)

Cultivate the spirit of scientific exploration

3

Comprehensiveness

Real experiment

Mandatory

Experiment 5

Freezing, single worm PCR and Genotyping

5

Comprehensiveness

Real experiment

Mandatory

Experiment 6

RNAi result analysis, fat staining and genetic cross (3)

Cultivate the spirit of scientific exploration

5

Comprehensiveness

Real experiment

Mandatory

Online   seminars

Asia Pacific Worm Meeting (online)

2

Experiment 7

Application of fluorescent protein in Biology (gene expression   regulation and protein localization in cells)

3

Comprehensiveness

Real experiment

Mandatory

Lecture 4

Experimental techniques and methods commonly used in the study of C. elegans

Cultivate   students' interest in science, scientific spirit and national pride

2

Experiment 8

C. elegans lab visit and microinjection

9

Comprehensiveness

Real experiment

Mandatory

Journal presentation and discussion

4

Total

50

6. Assessment methods   and requirements

Grading   plan: hundred-mark system; Score composition: lecture assignments (4 times,   accounting for 20%), experimental operation assessments (3 times, accounting   for 15%), experimental reports (8 times, accounting for 45%), literature   reading and presentation (1 time, accounting for 20%). Assessment criteria:   lecture homework and experimental reports: after the students hand it in, the   teacher scores according to the execution quality. Experimental operation   assessment: the students complete the specified experimental operation, and   the teacher scores according to the objective criteria of the experimental   results. Literature reading and presentation: the students read the specified   English literature, make slides and present, and the teacher scores according   to their understanding and presentation.

7.Textbooks/References

用途(教材；教辅),出版物名称,ISBN,出版年月,教材类别,主编

the use of book(textbooks;references),book   title, ISBN, year of publication, types , author

Self-compiled PowerPoint   slides

8.Major literature or related databases

Donald L Riddle et. al. C. elegans II，2nd edition, Cold Spring   Harbor Laboratory Press，1997

9.Website

10.Others

Filled out by:               Approved by:                Date:            July 1^st, 2022

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