厦 门 大 学
教 学 进 度 表
(2022-2023学年第2学期)
课程名称 细胞与显微技术实验 学分 3 上课系、专业、年级 生科院2022级
教师姓名 江良荣 职称 邮箱lrjiang108@xmu.edu.cn 电话 13599509420
周数 (按校历) |
各章节教学内容纲要 |
课时安排(学时) |
主讲人 |
备注 |
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授课 |
讨论 |
实验/上机 |
实践 |
其他 |
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3 |
相差显微镜与暗视野显微镜的使用 |
1 |
|
5 |
|
|
余娴文 靳全文 |
小测1次、作业1次 |
4 |
细胞荧光染色观察与荧光显微镜的使用;动物细胞线粒体的超活染色与油镜观察 |
1 |
|
5 |
|
|
余娴文 靳全文 |
小测1次、作业1次 |
5 |
细胞核与线粒体的分级分离(差速离心) |
1 |
|
5 |
|
|
靳全文 江良荣 |
小测1次、作业1次 |
6 |
兔血细胞计数及细胞大小的测量 |
1 |
|
5 |
|
|
靳全文 江良荣 |
小测1次、作业1次 |
7 |
叶绿体的分离与观察(密度梯度离心)(直接荧光与间接荧光的比较) |
1 |
|
5 |
|
|
靳全文 刘 敏 |
小测1次、作业1次 |
8 |
植物细胞骨架的观察 |
1 |
|
5 |
|
|
靳全文 刘 敏 |
小测1次、作业1次 |
9 |
细胞Barr body 的观察 |
1 |
|
5 |
|
|
刘 敏黄秋英 |
小测1次、作业1次 |
10 |
免疫荧光染色观察肿瘤细胞骨架 |
1 |
|
5 |
|
|
刘 敏黄秋英 |
小测1次、作业1次 |
11 |
细胞染色体的制备与染色 |
1 |
|
5 |
|
|
黄秋英 刘 敏 |
小测1次、作业1次 |
12 |
血红细胞通透性与细胞融合的观察 |
1 |
|
5 |
|
|
黄秋英 刘 敏 |
小测1次、作业1次 |
13 |
细胞凝集反应 |
1 |
|
5 |
|
|
黄秋英 江良荣 |
小测1次、作业1次 |
14 |
石蜡切片技术创新性实验 (一)取材(阿兹夫定喂养小鼠)、固定、洗涤、脱水 (二)透明、透蜡、包埋 |
1 |
|
5 |
|
|
江良荣叶 军 |
小测1次、作业1次 |
15 |
(三)切片、贴片;实验十四、冰冻切片技术 |
1 |
|
5 |
|
|
江良荣叶 军 |
小测1次、作业1次 |
16 |
(四)染色(分化与蓝化的组合分析)、封藏 |
1 |
|
5 |
|
|
江良荣叶 军 |
小测1次、作业1次 |
17 |
组织化学创新性实验——小鼠肝和胸腺糖元染色;Feulgen 反应 |
1 |
|
5 |
|
|
叶 军 余娴文 |
小测1次、作业1次 |
18 |
免疫组织化学创新性实验——小鼠脑和胸腺血管的DAB显色 |
1 |
|
5 |
|
|
叶 军 余娴文 |
小测1次、作业1次 |
19 |
复习、期末考试 |
|
|
|
|
|
|
期末考试 |
合计 |
16 |
|
80 |
|
|
|
|
制定者: 审定者: 制定时间: 年 月 日
Xiamen University
Teaching Schedule
(Academic year 2022-2023,Semester 2)
Course name Cell and microscopic techniques Credit 3 Dept., Class School of Life Sciences, 2021
Instructor name Liangrong Jiang Title Email lrjiang108@xmu.edu.cn Tel 13599509420
Week (University Calendar) |
Content |
Learning Hours |
|
Note |
||||
Lecture |
Tutorial |
Experiment |
Practical |
Others |
||||
3 |
The usage of Phase contrast and dark field microscope |
1 |
|
5 |
|
|
Xianwen Yu Quanwen Jin |
1 quiz and 1 assignment |
4 |
Fluorescence staining and the usage of fluorescence microscopy; Animal cell mitochondria vital staining and oil microscopic observation |
|
|
5 |
|
|
Xianwen Yu Quanwen Jin |
1 quiz and 1 assignment |
5 |
Fractionation of nuclei and mitochondria (differential centrifugation) |
1 |
|
5 |
|
|
Quanwen Jin Liangrong Jiang |
1 quiz and 1 assignment |
6 |
Cell count and size measurement of rabbit blood cells |
1 |
|
5 |
|
|
Quanwen Jin Liangrong Jiang |
1 quiz and 1 assignment |
7 |
Isolation and observation of chloroplasts (density gradient centrifugation) (Comparison of direct and indirect fluorescence) |
1 |
|
5 |
|
|
Quanwen Jin Min Liu |
1 quiz and 1 assignment |
8 |
Observation of cytoskeleton of plant cells |
1 |
|
5 |
|
|
Quanwen Jin Min Liu |
1 quiz and 1 assignment |
9 |
Observation of cell barr body |
1 |
|
5 |
|
|
Min Liu Qiuying Huang |
1 quiz and 1 assignment |
10 |
Observation of cytoskeleton by immunofluorescence |
1 |
|
5 |
|
|
Min Liu Qiuying Huang |
1 quiz and 1 assignment |
11 |
Preparation and staining of cell chromosome |
1 |
|
5 |
|
|
Qiuying Huang Min Liu |
1 quiz and 1 assignment |
12 |
Observation of the permeability and fusion of red blood cell |
1 |
|
5 |
|
|
Qiuying Huang Min Liu |
1 quiz and 1 assignment |
13 |
Cell agglutination |
1 |
|
5 |
|
|
Qiuying Huang Liangrong Jiang |
1 quiz and 1 assignment |
14 |
Paraffin sectioning (Innovation experiment) (1) specimen preparation (The mice were fed with the medicine, Azvudine), fixation , washing , dehydration (2) Clearing, infiltration and embedding |
1 |
|
5 |
|
|
Liangrong Jiang Jun ye |
1 quiz and 1 assignment |
15 |
(3) Sectioning; Frozen section technique (Innovation experiment) |
1 |
|
5 |
|
|
Liangrong Jiang Jun ye |
1 quiz and 1 assignment |
16 |
(4) Staining and storage (Innovation experiment) |
1 |
|
5 |
|
|
Liangrong Jiang Jun ye |
1 quiz and 1 assignment |
17 |
Histochemistry (Innovation experiment): Mouse liver and thymus glycogen staining (PAS reaction);Mouse liver and thymus DNA staining (Feulgenreaction) |
1 |
|
5 |
|
|
Jun ye Xianwen Yu |
1 quiz and 1 assignment |
18 |
Immunohistochemical assay (Innovation experiment):DAB staining of brain and thymus vessels in mice |
1 |
|
5 |
|
|
Jun ye Xianwen Yu |
1 quiz and 1 assignment |
19 |
review and final exam |
|
|
|
|
|
|
Final exam |
Total |
16 |
|
80 |
|
|
|
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Filled out by Approved by: Date: