厦 门 大 学
教 学 进 度 表
(2021-2022学年第3学期)
课程名称 生物技术实验 学分 2 上课系、专业、年级 生科院2019级
教师姓名 李勤喜,林舒勇 职称 教授/教授 邮箱liqinxi@xmu.edu.cn, linsy@xmu.edu.cn
电话13850006392,15985860458
周数 |
各章节教学内容纲要 |
课时安排(学时) |
主讲人 |
备注 |
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授课 |
讨论 |
实验/上机 |
实践 |
其他 |
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4 |
哺乳动物细胞培养 |
1 |
1 |
4 |
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李勤喜 |
|
4 |
细胞总RNA 的制备 |
1 |
1 |
4 |
|
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李勤喜 |
|
4 |
mRNA反转录成cDNA |
1 |
1 |
4 |
|
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林舒勇 |
|
4 |
PCR扩增目的基因 |
1 |
1 |
4 |
|
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林舒勇 |
|
4 |
哺乳动物细胞基因转染 |
1 |
1 |
4 |
|
|
林舒勇 |
|
4 |
1 |
2 |
9 |
|
|
林舒勇 |
|
|
5 |
Western 印迹法检测目的基因的表达 |
2 |
2 |
10 |
|
|
李勤喜 |
|
5 |
荧光蛋白作为报告基因研究蛋白的表达和定位 |
2 |
2 |
10 |
|
|
李勤喜 |
|
5 |
答疑 |
|
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李勤喜 林舒勇 |
|
合计 |
70 |
|
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制定者:李勤喜 审定者:王勤 制定时间:2022年5月28 日
Xiamen University
Teaching Schedule
(Academic year 2021-2022,Semester 3)
Course name Biotechnique Experiments Credit 2 Dept., Class school of life sciences, 2019
Instructor name Qinxi Li, Shuyong Lin Title Professor/ Professor
Email liqinxi@xmu.edu.cn, linsy@xmu.edu.cn Tel 13850006392, 15985860458
Week (University Calendar) |
Content |
Learning Ho rs |
Instructor |
Note |
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Lecture |
Tutorial |
Experiment |
Practical |
Others |
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1 |
Mammalian cell culture |
1 |
1 |
4 |
|
|
Qinxi Li |
|
1 |
|
|
1 |
4 |
|
|
Qinxi Li |
|
1 |
Reverse transcription |
1 |
1 |
4 |
|
|
Shuyong Lin |
|
1 |
PCR technology |
1 |
1 |
4 |
|
|
Shuyong Lin |
|
1 |
Gene transfection in mammalian cell |
1 |
1 |
4 |
|
|
Shuyong Lin |
|
1 |
Study of proteins interactions using co-immunoprecipitation |
1 |
2 |
9 |
|
|
Shuyong Lin |
|
2 |
Analysis of gene expression by Western blot |
2 |
2 |
10 |
|
|
Qinxi Li |
|
2 |
Study of proteins expression and co-localization by using fused fluorescent proteins proteins
|
2 |
2 |
10 |
|
|
Qinxi Li |
|
2 |
Review |
|
|
|
|
|
Qinxi Li Shuyong Lin |
|
Total |
70 |
|
|
|
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Filled out by: Qinxi Li Approved by: Qin Wang Date: 2022.5.28