厦 门 大 学
教 学 进 度 表
(2021－2022学年第3学期)
课程名称 模式生物秀丽线虫：入门与实践 学分2 上课系、专业、年级 生科院2019级、2020级
教师姓名 俞勇 职称 副教授 邮箱 yuy@xmu.edu.cn 电话18850155283
周数 (按校历) |
各章节教学内容纲要 |
课时安排（学时） |
主讲人 |
备注 |
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授课 |
讨论 |
实验/上机 |
实践 |
其他 |
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第4周周一上午 |
秀丽线虫作为模式生物的历史回顾及优势 |
2 |
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|
|
|
俞勇 |
作业1 |
秀丽线虫培养及基本操作 |
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|
3 |
|
|
俞勇 |
实验报告1 |
|
第4周周二 |
秀丽线虫突变体辨识、同步化及遗传杂交(1) |
|
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5 |
|
|
俞勇 |
实验考核2 实验报告2 |
第4周周三上午 |
秀丽线虫的形态与解剖结构 |
2 |
|
|
|
|
俞勇 |
作业2 |
第4周周三下午 |
秀丽线虫发育过程及形态、解剖结构观察 |
|
|
3 |
|
|
俞勇 |
实验考核3 |
第4周周四上午 |
秀丽线虫的基因与发育等内容 |
2 |
|
|
|
|
罗时成 |
作业3 |
第4周周四下午 |
秀丽线虫的RNAi及遗传杂交(2) |
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3 |
|
|
俞勇 |
实验报告4 |
第4周周五 |
冻存，Single worm PCR and Genotyping |
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|
5 |
|
|
俞勇 |
实验报告5 |
RNAi结果分析和脂肪染色，遗传杂交(3) |
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|
5 |
|
|
俞勇 |
实验报告6 |
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第5周周二上午 |
亚洲秀丽线虫大会线上报告
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2 |
|
|
|
俞勇 |
|
第5周周二下午 |
荧光蛋白在生物学中的应用(基因表达调控及蛋白质在细胞中定位研究) |
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|
3 |
|
|
俞勇 |
实验报告7 |
第5周周三上午 |
秀丽线虫研究中常用的实验技术及方法 |
2 |
|
|
|
|
|
作业4 |
第5周周四-五上午 |
文献报告与讨论 |
2 |
2 |
|
|
|
俞勇 |
|
第5周周三-五下午 |
|
|
9 |
|
|
俞勇 |
实验报告8 |
|
合计 |
10 |
4 |
36 |
|
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制定者： 审定者： 制定时间：2022年7月10日
Teaching Schedule
(Academic year 2021－2022, Semester 3)
Course nameThe model organism Caenorhabditis elegans: introduction and practice Credit 2 Dept., Class School of Life Sciences, 2019, 2020
Instructor name Yong Yu Title Associate Prof. Email yuy@xmu.edu.cn Tel 18850155283
Week (University Calendar) |
Content |
Learning Hours |
|
Note |
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Lecture |
Tutorial |
Experiment |
Practical |
Others |
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4th Week, Mon. AM. |
Historical review and advantages of C. elegans as a model organism |
2 |
|
|
|
|
Yong Yu |
Assignment 1 |
4th Week, Mon. PM. |
Culture and basic operation of C. elegans |
|
3 |
|
|
Yong Yu |
Experimental examination 1; Experimental report 1 |
|
4th Week, Tue. |
Mutant identification, synchronization and genetic cross of C. elegans (1) |
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|
5 |
|
|
Yong Yu |
Experimental examination 2; Experimental report 2 |
4th Week, Wed. AM. |
Morphology and anatomical structure of C. elegans |
2 |
|
|
|
|
Yong Yu |
Assignment 2 |
4th Week, Wed. PM. |
Observation on developmental process, morphology and anatomical structure of C. elegans |
|
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3 |
|
|
Yong Yu |
Experimental examination 3; Experimental report 3 |
4th Week, Thu. AM |
Genes and development of C. elegans |
2 |
|
|
|
|
SJ Lo |
Assignment 3 |
4th Week, Thu. PM |
C. elegans RNAi and genetic cross (2) |
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3 |
|
|
Yong Yu |
Experimental report 4 |
4th Week, Fri. |
Freezing, single worm PCR and Genotyping |
|
|
5 |
|
|
Yong Yu |
Experimental report 5 |
Mon. |
RNAi result analysis, fat staining and genetic cross (3) |
|
|
5 |
|
|
Yong Yu |
Experimental report 6 |
5th Week, Tue. AM |
Asia Pacific Worm Meeting, online seminar |
|
2 |
|
|
|
Yong Yu |
|
5th Week, Tue. PM |
Application of fluorescent protein in Biology (gene expression regulation and protein localization in cells) |
|
|
3 |
|
|
Yong Yu |
Experimental report 7 |
5th Week, Wed. AM |
Experimental techniques and methods commonly used in the study of C. elegans |
2 |
|
|
|
|
Yong Yu |
Assignment 4 |
Thu to Fri. AM |
Journal presentation and discussion |
2 |
2 |
|
|
|
Yong Yu |
|
5th Week, Wed. to Fri. PM |
C. elegans lab visit and microinjection |
|
|
9 |
|
|
Yong Yu |
Experimental report 8 |
Total |
10 |
4 |
36 |
|
|
|
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Filled out by Approved by: Date: July 10th, 2022
